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                                                                  
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                                             	                                                                        
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            7                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
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  Ÿ<?xml version="1.0"?><Features nextValidID="14"><Feature recentID="0" name="ubiquitin" directionality="1" translationMW="8593.06" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1113" maxFusedRunOn="1113" detectionMode="exactProteinMatch"><Segment range="883-1110" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;human ubiquitin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="13" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3981-3997" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1405-1421" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1381-1397" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="1474-1495" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1113" maxFusedRunOn="1113" detectionMode="exactProteinMatch"><Segment range="832-858" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment name="-10" range="1429-1435" color="#ffffff" type="standard"/><Segment range="1436-1453" color="#ffffff" type="standard"/><Segment name="-35" range="1454-1459" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; 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  ]<Notes>
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<Type>Synthetic</Type>
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<LastModified UTC="7:24:38">2025.12.4</LastModified>
<CreatedBy>Huanghua</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
</Notes>
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ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
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  ]<Notes>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2016.6.28</Created>
<LastModified UTC="7:23:19">2025.12.4</LastModified>
<CreatedBy>Huanghua</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
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  �        �±:6>4,§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDì©!|´løÏosŠ¡ûÙwÀ|oO¼äA¼'‘áUôT¥à”©.ª.©«BüÂ#VÕŽûH£êzä>'"ž¸îU8ÁhŸÌßbg¯Fœî˜=�Å¦5n²‡ÃAößäBý!ßlEÈPõÐeD¿þMíº�M8`ó»‡�vôTK_ã~ï>9{Ÿ0ó oGa”gha*WÝy4…Uc©¨…j!Žhêj%ö™N5•Ô¥×]hÚÚ¦*Ð­ ˆ*«û‘,n469:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Â
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ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô  r  �  “  X  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  \<Notes>
<UUID>0b6ba793-2268-4b25-97e2-53bfe732df5a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2016.6.28</Created>
<LastModified UTC="7:22:3">2025.12.4</LastModified>
<CreatedBy>Huanghua</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
</Notes>
  	<StrandColors><TopStrand><ColorRange range="882..1109" colors="black"/><ColorRange range="1110..1112" colors="blue"/></TopStrand><BottomStrand><ColorRange range="882..1109" colors="black"/><ColorRange range="1110..1112" colors="blue"/></BottomStrand></StrandColors>  !é     
  �        �±:6>4,§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDì©!|´løÏosŠ¡ûÙwÀ|oO¼äA¼'‘áUôT¥à”©.ª.©«BüÂ#VÕŽûH£êzä>'"ž¸îU8ÁhŸÌßbg¯Fœî˜=�Å¦5n²‡ÃQößäBý!ßlEÈPôÐeD¿þMíº�M8`ó»‡�vôTK_ã~ï>9{Ÿ0ó oGa”gha*WÝy4…Uc©¨…j!Žhêj%ö™N5•Ô¥×]hÚÚ¦*Ð­ ˆ*«û‘,n469:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Â
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ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô  r  Æ  È  X  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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<Created UTC="16:0:0">2016.6.28</Created>
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  �        �±:6>4,§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDì©!|´løÏosŠ¡ûÙwÀ|oO¼äA¼'‘áUôT¥à”©.ª.©«BüÂ#VÕŽûH£êzä>'"ž¸îU8ÁhŸÌßbg¯Fœî˜=�Å¦5n²‡ÃQößäBý!ßlEÈ@õÐeD¿þMíº�M8`ó»‡�vôTK_ã~ï>9{Ÿ0ó oGa”gha*WÝy4…Uc©¨…j!Žhêj%ö™N5•Ô¥×]hÚÚ¦*Ð­ ˆ*«û‘,n469:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Â
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ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô  r  À  Â  X  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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<UUID>0b6ba793-2268-4b25-97e2-53bfe732df5a</UUID>
<Type>Synthetic</Type>
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<Created UTC="16:0:0">2016.6.28</Created>
<LastModified UTC="7:16:39">2025.12.4</LastModified>
<CreatedBy>Huanghua</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
</Notes>
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  �        �±:6>4,§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDì©!|´løÏosŠ¡ûÙwÀ|oO¼äA¼'‘áUôT¥à”©.ª.©«BüÂ#VÕŽûH£êzä>'"ž¸îU8ÁhŸÌßbg¯Fœî˜=�Å¦5n²‡ÃQößäBý!ßlEÈPõÐdD¿þMíº�M8`ó»‡�vôTK_ã~ï>9{Ÿ0ó oGa”gha*WÝy4…Uc©¨…j!Žhêj%ö™N5•Ô¥×]hÚÚ¦*Ð­ ˆ*«û‘,n469:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Â
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ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
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<LastModified UTC="7:13:39">2025.12.4</LastModified>
<CreatedBy>Huanghua</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
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<SequenceClass>UNA</SequenceClass>
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<CustomMapLabel>pCMV-HA-Ub(rat)-K48R</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2016.6.28</Created>
<LastModified UTC="4:44:32">2026.3.26</LastModified>
<CreatedBy>Huanghua</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>DH5Î±â„¢</TransformedInto>
</Notes>
  Y      K         
?????                                                                                                       ÿ d      DO  d               E              ÿÿÿÿYô? P                                                                                                                                                             	<StrandColors><TopStrand><ColorRange range="882..1109" colors="black"/><ColorRange range="1110..1112" colors="blue"/></TopStrand><BottomStrand><ColorRange range="882..1109" colors="black"/><ColorRange range="1110..1112" colors="blue"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
